--- title: DAC Chromatography Columns | LP, MP & HP | Peak Biotech description: Peak Biotech DAC columns for GMP biopharmaceutical production. LP (10 bar), MP (30 bar) and HP (100 bar) pressure classes. Oil-free, mobile design. Danish-manufactured. image: https://peakbiotech.com/hubfs/Column_mobile_800.jpg --- [Skip to content](https://peakbiotech.com/columns#main-content) - [Insights](https://peakbiotech.com/insights) - [FAQs](https://peakbiotech.com/chromatography-frequently-asked-questions) Close - [Columns](https://peakbiotech.com/columns) - [Systems](https://peakbiotech.com/systems) - [Accessories](https://peakbiotech.com/accessories) - [About](https://peakbiotech.com/about) SEARCH CONTACT PHONE HOTLINE: [+4570276279](tel:+4570276279)E-MAIL: [contact@peakbiotech.com](mailto:contact@peakbiotech.com) [Contact](https://peakbiotech.com/contact?hsLang=en) [![Peak Biotech logo RGB - small placement](https://peakbiotech.com/hs-fs/hubfs/Peak%20Biotech%20logo%20RGB%20-%20small%20placement.png?width=1238&height=561&name=Peak%20Biotech%20logo%20RGB%20-%20small%20placement.png)![Peak Biotech logo RGB - small placement - white](https://peakbiotech.com/hs-fs/hubfs/Peak%20Biotech%20logo%20RGB%20-%20small%20placement%20-%20white.png?width=1238&height=561&name=Peak%20Biotech%20logo%20RGB%20-%20small%20placement%20-%20white.png)](https://peakbiotech.com?hsLang=en) - [Columns](https://peakbiotech.com/columns) - [Systems](https://peakbiotech.com/systems) - [Accessories](https://peakbiotech.com/accessories) - [About](https://peakbiotech.com/about) PHONE HOTLINE: [+4570276279](tel:+4570276279)E-MAIL: [contact@peakbiotech.com](mailto:contact@peakbiotech.com) [Contact](https://peakbiotech.com/contact?hsLang=en) ![Peak Biotech chromatography columns GMP production facility](https://peakbiotech.com/hs-fs/hubfs/Hero_2000.jpg?width=300&name=Hero_2000.jpg) # Chromatography Columns ##### **Three pressure classes. Two design platforms. One engineering philosophy.** [Talk to an Engineer](https://peakbiotech.com/contact?hsLang=en) ## LP, MP and HP DAC Columns Peak Biotech designs and manufactures chromatography columns for low, medium, and high pressure chromatography. Two design platforms cover the three pressure classes: **• A shared LP and MP platform up to 30 bar** **• A dedicated HP platform up to 100 bar ** Each column is built around the process it will run, with the documentation, materials, and lifecycle support a GMP facility expects to find on day one and twenty years from now. Oil-free Internal Hydraulics 20% ethanol replaces hydraulic oil. The compression mechanism sits inside the column body, removing the external hydraulic frame and the contamination risk that comes with oil in a cleanroom. Controlled Axial Compression Pressure distributes uniformly across the resin bed. Bed density is reproducible from one pack to the next and stays stable across production campaigns. Fully Mobile Design Internal hydraulics remove the overhead compression frame. The column stands on its own, rolls on castors, and moves with standard facility handling equipment. No fixed installation. No dedicated floor space. Retrofit-ready from day one. External Leak Visibility The atmospheric cavity between hydraulic and process sides means seal failures become externally visible before they reach the resin bed or the product. Three operating windows. Two design platforms. ## Three Pressure Classes Pressure class follows the process. LP columns sit at protein capture and intermediate purification. MP columns handle polishing and resin chemistries that require firmer compression. The LP and MP columns share a single design platform: the same body, the same hydraulics, the same handling interfaces.  HP columns run high-resolution separations where bed integrity at elevated pressure determines yield, and the HP platform is a dedicated mechanical design built around that pressure window. The hydraulic principle, the documentation package, and the lifecycle support are consistent across both platforms. | | **Low Pressure Columns** | **Medium Pressure Columns** | **High Pressure Columns** | | --- | --- | --- | --- | | **Design platform**​ | Shared LP & MP platform​ | Shared LP & MP platform​ | Dedicated HP platform​ | | **Pressure window**​ | Up to 10 bar​ | Typically 10 to 30 bar​ | Up to 100 bar depending on configuration​ | | **Typical applications**​ | Capture and intermediate purification using agarose based chromatography media for monoclonal antibodies and recombinant biologics​ | Polishing and intermediate purification using higher pressure agarose and polymer based chromatography media​ | High resolution preparative HPLC, silica based separations, peptides, oligonucleotides and small molecule APIs​ | | **Internal diameter**​ | 200 to 1800 mm​ | 200 to 1200 mm​ | 100 to 800 mm​ | | **Bed height (packed resin)**​ | 100 to 500 mm​ | 150 to 600 mm​ | 200 to 450 mm​ | | **Hydraulic principle**​ | Internal hydraulic DAC system using ethanol-water hydraulic fluid​ | Internal hydraulic DAC system using ethanol-water hydraulic fluid​ | Internal hydraulic DAC system using ethanol-water hydraulic fluid​ | | **Wetted materials**​ | SS 316L​ | SS 316L​ | SS 316L as standard, Duplex stainless steel optional​ | | **Seal materials**​ | PEHD, PTFE​ | PEEK, PTFE​ | PEEK, PTFE​ |   The packing principle behind every column ## Dynamic Axial Compression Every column we build is packed by the same principle: Dynamic Axial Compression.​ The resin enters the column as a slurry; a liquid suspension pumped in through the bottom. Once the slurry is in, the piston moves down. Hydraulic pressure drives it at a controlled speed, compressing the resin evenly across the full cross section of the column. The packing buffer drains out through the bottom as the bed forms. When the target bed height and compression are reached, the hydraulic chamber closes and the pressure is maintained.​ The hydraulic fluid is 20% ethanol. It sits inside the column body, separated from the process side by an atmospheric cavity. There is no oil and no hydraulic lines running outside the column. If a seal were to fail, the leak would appear on the outside of the column before it could reach the resin or the product.​ Flow distribution is handled by a specially designed distributor at both the top and bottom of the column. This ensures that the mobile phase spreads evenly across the bed during both packing and operation.​ The result is a bed that is reproducible. The same piston speed, the same flow rate, the same bed height every time the column is packed, whether it is 200 mm or 1800 mm in diameter, and whether it is the first pack or the fiftieth. [Talk to an Engineer](https://peakbiotech.com/contact?hsLang=en) ![Ethanol-based hydraulic system (no oil) - column](https://147630852.fs1.hubspotusercontent-eu1.net/hub/147630852/hubfs/Column%20in%20detail%201.png?width=300&name=Column%20in%20detail%201.png) Built for the room it will actually live in ## Compact Design and Cleanability Internal hydraulics remove the overhead compression frame that traditional DAC columns require. The result is a column with significantly reduced overall height, smooth external surfaces, and few wipe areas. Most models are fully mobile on castors. Larger columns are designed to be moved with standard handling equipment. Cleaning is faster because there are no external hydraulic oil lines, no overhead hoses, and few accumulation points. Visual QA inspection is straightforward. The external geometry was designed with the cleanroom operator in mind. ![Low-pressure DAC column 800mm diameter — Peak Biotech](https://147630852.fs1.hubspotusercontent-eu1.net/hub/147630852/hubfs/LPLC_800.png?width=300&name=LPLC_800.png) Reduced Overall Height No external compression frame, no overhead hydraulic cylinder. Ceiling height becomes a non-issue in retrofit projects. Designed for Movement Most models roll on castors. Larger columns include lifting interfaces for forklift or overhead crane. Ergonomic handling accessories available as options. External Cleanability Smooth surfaces, minimal external components, no exposed hydraulic fluid lines. Faster wipe down, easier visual inspection, lower operator workload. **Cleanability comparison** | **Design feature** | **Peak Biotech column** | | --- | --- | | Hydraulic system | Internal | | Hydraulic fluid exposure | No external fluid lines | | External components | Minimal | | Surface geometry | Smooth | | Cleaning access | Easy wipe down | | Risk of residues | Very low |   ## From Column to Full Production Capability A column is the mechanical core of the step. Reproducible results across campaigns depend on the systems and support built around it. ### Handling and Packing Equipment Safe handling, reproducible packing, and controlled maintenance depend on the equipment around the column. Slurry preparation units, bottom handling carts, rotation stands, and bed height rulers are part of the engineered offering and are designed alongside the column they serve. ![Chromatography Column](https://147630852.fs1.hubspotusercontent-eu1.net/hub/147630852/hubfs/Screenshot%202026-07-14%20at%2008.53.36.png?width=300&name=Screenshot%202026-07-14%20at%2008.53.36.png) ### Lifecycle Support The economics of a chromatography column happen across the lifetime of the asset rather than at the moment of purchase. Spare parts, qualification documentation, operator training, and on-site assistance are part of the standard relationship and are delivered globally under a structured zone-based support model. ![PLC\_800](https://147630852.fs1.hubspotusercontent-eu1.net/hub/147630852/hubfs/PLC_800.jpg?width=300&name=PLC_800.jpg) ## Talk to an Engineer [![Peak Biotech logo RGB - small placement - white](https://147630852.fs1.hubspotusercontent-eu1.net/hubfs/147630852/Peak%20Biotech%20logo%20RGB%20-%20small%20placement%20-%20white.svg)](https://peakbiotech.com/?hsLang=en) Peak Biotech A/S manufactures chromatography equipment from pilot to production process scale for the pharmaceutical and biotech industry. - Equipment - [Columns](https://peakbiotech.com/columns) - [Systems](https://peakbiotech.com/systems) - [Accessories](https://peakbiotech.com/accessories) - Resources - [Insights](https://peakbiotech.com/insights) - [Frequently Asked Questions](https://peakbiotech.com/chromatography-frequently-asked-questions) SUBSCRIBE © 2026 Peak Biotech A/S - [Impressum](https://peakbiotech.com/impressum) - [Terms & Conditions](https://peakbiotech.com/terms-and-conditions) - [Privacy Policy](https://peakbiotech.com/privacy-policy) - [Cookie Declaration](https://peakbiotech.com/cookie-declaration) ```json { "@context" : "https://schema.org", "@type" : "Organization", "address" : { "@type" : "PostalAddress", "addressCountry" : "Denmark", "addressLocality" : "Kvistgaard", "addressRegion" : "Helsingør", "postalCode" : "3490", "streetAddress" : "Bøgeskovvej 18A" }, "email" : "contact@peakbiotech.com", "knowsLanguage" : "en", "logo" : { "@type" : "ImageObject", "url" : "https://147630852.fs1.hubspotusercontent-eu1.net/hubfs/147630852/Peak%20Biotech%20logo%20RGB%20-%20small%20placement.svg" }, "name" : "Peak Biotech A/S", "telephone" : "+4570276279", "url" : "https://peakbiotech.com/columns" } ```